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KMID : 0613820080180081059
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Journal of Life Science
2008 Volume.18 No. 8 p.1059 ~ p.1065
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Pcp-2 Interacts Directly with Kinesin Superfamily KIF21A Protein
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Park Hye-Young
Kim Sang-Jin
Yea Sung-Su
Jang Won-Hee
Lee Sang-Kyung
Park Yeong-Hong
Jung Yong-Wook
Moon Il-Soo
Kim Moo-Seong
Seog Dae-Hyun
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Abstract
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KIF21A is a member of the Kinesin superfamily proteins (KIFs), which are microtubule-dependent molecular motors, anterograde axonal transporters of cargoes. Recently, congenital fibrosis of the extraocular muscles 1 (CFEOM1) has been shown to result from a small number of recurrent heterozygous missense mutations of KIF21A. CFEOM1 results from the inability of mutated KIF21A to successfully deliver cargoes to the development of the occulo-motor neuron or neuromuscular junction. Here, we used an yeast two-hybrid system to identify a protein that interacts with the WD-40 repeat domain of KIF21A and found a specific interaction with Purkinje cell protein-2 (Pcp-2), a small protein also known as L7. Pcp-2 protein bound to the WD-40 domain of KIF21A and KIF21B but not to other KIFs in yeast two-hybrid assays. In addition, this specific interaction was also observed in the glutathione S-transferase pull-down assay. An antibody to Pcp-2 specifically co-immunoprecipitated KIF21A associated with Pcp-2 from mouse brain extracts. These results suggest that Pcp-2 may be involved in the KIF21A-mediated transport as a KIF21A adaptor protein.
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KEYWORD
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Kinesin, Purkinje cell protein-2, adaptor proteins, WD-40 repeats
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